Everything about Aesculin totally explained
Aesculin is a
glucoside that naturally occurs in the
horse chestnut Aesculus hippocastanum and in
daphnin (the dark green resin of
Daphne mezereum).
Medical uses
Aesculin is used in a microbiology laboratory to aid in the identification of bacterial species (especially
Enterococci).
Aesculin hydrolysis test
Aesculin is incorporated into agar with
ferric citrate and bile salts (
bile aesculin agar). Hydrolysis of the aesculin forms aesculetin (6,7-dihydroxy
coumarin) and
glucose. The aesculetin forms dark brown or black complexes with ferric citrate, allowing the test to be read.
Streak the bile aesculin agar and incubate at 37°C for 24 hours. The presence of a dark brown or black halo indicates that the test is positive. A positive test can occur with
Enterococcus,
Aerococcus and
Leuconostoc. Aesculin will
fluoresce under long wave
ultraviolet light (360): hydrolysis of aesculin results in loss of this fluorescence.
Enterococcus will often flag positive within four hours of the agar being inoculated.
Further Information
Get more info on 'Aesculin'.
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